This course is aimed at Technicians and Scientists interested in the generation of transgenic mice that have basic experience with eukaryotic cells and microscopy.
About 30% of the course program covers lectures and tutorials on generating transgenic mice and corresponding microscopy and laser techniques by experts in the field. The rest of the time will focus on hands on practical work organised in small groups. These practical will address the following topics: injection of DNA into the pronucleus of fertilized mouse oocytes, embryonic stem cell transfer into blastocysts, laser-assisted stem cell transfer into 8-cell stage embryos, and microscopy training.
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